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Image Search Results
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Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Detailed methods are provided in the online version of this paper and include the following: table ft1 table-wrap mode="anchored" t5 REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer
Techniques: Recombinant, Lysis, shRNA, Plasmid Preparation, Software